Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection
نویسندگان
چکیده
An impedimetric immunosensor for fumonisin B₁ (FB₁) was developed from a poly(2,5-dimethoxyaniline)-multi-walled carbon nanotube (PDMA-MWCNT) composite on the surface of glassy carbon electrode (GCE). The composite was prepared electrochemically and characterized using cyclic voltammetry. The preparation of the FB₁ immunosensor involved the drop-coating of a bovine serum albumin mixture of the anti-fumonisin antibody (anti-Fms) onto the composite polymer-modified GCE. The electrochemical impedance spectroscopy (EIS) responses of the FB₁ immunosensor (GCE/PDMA-MWCNT/anti-Fms) have a linear range of 7 to 49 ng·L-1, and the corresponding sensitivity and detection limits are 0.272 kΩ L·ng-1 and 3.8 pg·L-1, respectively. The limit of detection of the immunosensor for certified corn sample (i.e., certified reference material) is 0.014 ppm FB₁, which is in excellent agreement with the value published by the vendors and significantly more accurate than that obtained with enzyme-linked immunosorbent assay (ELISA).
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A Fumonisins Immunosensor Based on Polyanilino-Carbon Nanotubes Doped with Palladium Telluride Quantum Dots
An impedimetric immunosensor for fumonisins was developed based on poly(2,5-dimethoxyaniline)-multi-wall carbon nanotubes doped with palladium telluride quantum dots onto a glassy carbon surface. The composite was assembled by a layer-by-layer method to form a multilayer film of quantum dots (QDs) and poly(2,5-dimethoxyaniline)-multi-wall carbon nanotubes (PDMA-MWCNT). Preparation of the electr...
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